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چکیده
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BACKGROUND: ovarian transplantation is a successful method of maintaining fertility in young women, however, the occurrence of ischemic-reperfusion can affect the endocrine activity of Transplanted tissue. Injectable platelet rich fibrin (i-PRF) is a liquid formulation of platelet rich fibrin (PRF) without the use of anti-coagulants. i-PRF is bioscaffold that contains leukocytes, circulating stem cells, platelets and growth factors. All of these factors are able to affect the growth and proliferation of granulosa cells and corpus luteum and thus cause the resumption of ovarian hormonal activity after transplantation. OBJECTIVE: We aimed to investigate the effect of i-PRF bioscaffold on the serum level of progesterone and estradiol following mouse ovarian tissue transplantation. METHODS: 18 famale Mice were divided into groups: control, autograft + saline (whole ovarian tissue transplanted in the gluteus superficialis muscle, saline directly injected into it), autograft + i-PRF (whole ovarian tissue transplanted in the gluteus superficialis muscle, i-PRF was directly injected into it ). 28 days after ovary transplantation, serum concentrations of progesterone and estradiol were assayed. Statistical analysis was done with one-way ANOVA and Tuckey’s test and the means were considered significantly different at p-value < 0.05. RESULTS: the serum level of progesterone and estradiol in the control group increased significantly compared to the other groups, while it showed a significant increase in the autograft + i-PRF group compared to the autograft group (p < 0.05). CONCLUSION: For the first time, our results showed that Injection of i-PRF into the graft site could reduce ischemic-reperfusion injury and improve ovarian tissue hormonal activity.
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