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چکیده
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BACKGROUND: Ovarian transplantation can be an effective technique to restore fertility in women with cancer who undergo radio/chemotherapy. however, this method also faces limitations such as ischemic-reperfusion injure and decreased blood supply after transplantation. Ovarian dysfunction and destruction of follicles lead to loss of reproductive and endocrine activities. Injectable platelet rich fibrin (i-PRF) is a liquid formulation of platelet rich fibrin (PRF) without the use of anti-coagulants Which also has anti-inflammatory properties. i-PRF is bioscaffold that contains leukocytes, circulating stem cells, platelets and growth factors. OBJECTIVE: In the present study, we investigated the effect of i-PRF bioscaffold on the total volume of the ovary, the volume of the cortex and medulla and Number of types of follicles following mouse ovarian tissue transplantation. METHODS: 18 Mice were randomly divided into: control, autograft + saline (whole ovarian tissue transplanted in the gluteus superficialis muscle, saline directly injected into it), autograft + i-PRF (whole ovarian tissue transplanted in the gluteus superficialis muscle, i-PRF was directly injected into it ). 28 days after ovary transplantation, the total volume of the ovary, the volume of the cortex and medulla and Number of types of follicles were estimated. Statistical analysis was done with one-way ANOVA and Tuckey’s test and the means were considered significantly different at p-value < 0.05. RESULTS: Results: in the autograft group, the total volume of the ovary, the volume of the cortex and medulla and Number of types of follicles decreased significantly compared to the control, while all these parameters showed a significant increase in the autograft + i-PRF group compared to the autograft group (p < 0.05). CONCLUSION: Our data showed that the use of i-PRF bioscaffold improves ovarian function after transplantation by reducing ischemic reperfusion injury.
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