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چکیده
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Introduction: Despite the beneficial effects of antibiotics in preventing the growth of bacteria and disease, these compounds can also damage host cells. Antibiotics damage mitochondria by reducing ATP, loss of respiratory capacity, oxidative stress due to ROS accumulation, and induction of cell death, and greatly contribute to the pathogenesis and progression of neurodegenerative diseases, including brain aging and Alzheimer's disease. Bacteriophages, on the other hand, are able to kill bacteria but have no effect on eukaryotic cells. in the present study, the therapeutic effect of colistin antibiotic and lytic bacteriophage against Pseudomonas aeruginosa on memory and learning was compared. Materials and Methods: In this experimental study, adult male Wistar rats weighing 250-220 g were divided into control (burn), phage lytic treatment (100 μl body weight) and colistin antibiotic treatment (1,000,000 international units - body weight) groups. In all groups, after anesthesia, third degree burns were created in the lumbar region of the animals. In the two groups of phage and antibiotic treatment, 100 μl of Pseudomonas was diluted to half McFarland at the burn site and performed after one hour of treatment. Bacteriophage and antibiotic treatment with the mentioned doses were performed intraperitoneally for 6 consecutive days. At the end of the treatment, passive avoidance test and new object detection test were used to assess memory. Results: In passive avoidance test, injection of colistin significantly reduced memory recall compared to the control group (P <0.05). While phage treatment had no significant effect on passive avoidance memory compared to the control group (P> 0.05). In the new object detection test, there was a significant difference in memory assessment between the antibiotic treatment group and the control group (P <0.05). While in the phage treatment group, there was no significant difference in the detection of a new object with the control group
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