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چکیده
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Today, most studies have focused on the solid phase in adsorption processes and the effect of its properties on the performance of the fluidized-bed systems. In this research, engineered microsphere agarose adsorbent was generated through a three-phase emulsion method that used to adsorb of the protein nanoparticles from a water-soluble solution in a fluidized-bed system. Moreover, Cibacron Blue 3GA dye ligand was immobilized onto the engineered microsphere agarose beads to adsorb lactoferrin as a model protein. A scanning electron microscope showed that the shape of engineered microsphere agarose adsorbents is spherical, with the size distribution range of 50-250 µm. Also, the porosity of the adsorbents defined around 90%, and the wet density was close to 2.6 g/mL. Then, to compare the performance of the engineered microsphere agarose adsorbents in a fluidized-bed system, the Cibacron Blue 3GA dye ligand was immobilized on the Streamline™ commercial adsorbent, and compared at the same conditions. In batch adsorption, the results of lactoferrin protein nanoparticle adsorption showed a higher dynamic binding capacity of engineered microsphere agarose adsorbent with Cibacron Blue 3GA. Also, the results demonstrated that most of the adsorption process occurred in the first half-hour, which is a very suitable time for a fluidized-bed system. Adsorption equilibrium experimental data were evaluated with isothermal adsorption models of Langmuir and Freundlich. Based on these results, Langmuir’s model better describes the data, and the maximum of adsorption was close to 45.3 mg/ml adsorbent. The fluidized-bed system adsorption showed that engineered adsorbents gained a sound breakthrough performance at high flow velocity and upper dynamic binding capacity compared to commercial adsorbents. The results indicated that in a constant liquid velocity as the adsorbent density was increased, the bed expansion decreased and the axial mixing coefficient increased. The dynamic bindin
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