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چکیده
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This study aimed to evaluate the effects of chromium propionate and vitamin C on the immune response of broiler chickens under heat stress conditions. A total of 360 one-day-old male Ross 308 broiler chicks were randomly assigned to six treatments, with six replications (12 chicks per replication) in a completely randomized design. The treatments included: a basal diet under normal temperature conditions (TN-CON), a basal diet under heat stress conditions (HS-CON), a basal diet supplemented with chromium propionate under heat stress (HS-CP), a basal diet supplemented with vitamin C under heat stress (HS-VC), and a basal diet supplemented with both chromium propionate and vitamin C under heat stress (HS-CC). The results showed that treatments containing chromium propionate (HS-CP and HS-CC) significantly increased relative spleen weight, total antibody titer, and the primary IgG titer (day 21) against SRBC compared to the HS-CON group (P < 0.05), which was similar to the TN-CON group. Additionally, all experimental treatments resulted in increased relative thymus weight and higher secondary antibody titers against SRBC compared to the HS-CON group (P < 0.05). The antibody titer against the Newcastle disease vaccine on days 28 and 35 was significantly higher in all experimental treatments, especially in the HS-CC group, compared to the HS-CON group (P < 0.05). However, relative bursa weight and primary and secondary IgM titers were unaffected by the experimental treatments. These findings suggest that chromium propionate and vitamin C positively influence the immune response of broiler chickens under heat stress conditions.
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