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چکیده
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This study aimed to evaluate the effects of phytase (PHY) and a feed enzyme complex (FEC) on blood parameters in broiler chickens fed standard (SN) and low-nutrient (LN) diets. The experiment utilized a 3×2 factorial design, including two diet types (high-quality and low-quality) and three enzyme treatments (no enzyme, phytase, and phytase + carbohydrase). A total of 900 male Ross 308 broiler chicks were assigned to six treatments with six replications, each containing 25 chicks. The results showed that the LN diet significantly reduced serum triglycerides and total protein compared to the SN diet (P < 0.05). Additionally, chickens fed the LN diet exhibited a significant decrease in aspartate transaminase (AST) activity (P < 0.05) and a downward trend in alanine transaminase (ALT) activity (P < 0.1) relative to the SN-fed groups. The inclusion of PHY in the diet led to a significant reduction in alkaline phosphatase (ALP) activity and an increase in parathyroid hormone (PTH) concentration (P < 0.05). However, statistical analysis revealed that neither the FEC nor the interaction effects of diet × PHY and diet × FEC significantly impacted any blood parameters, including triglycerides, cholesterol, total protein, AST, ALT, calcium, phosphorus, ALP, PTH, or calcitonin levels (P > 0.05). This study suggests that low-nutrient diets may affect the metabolic health of broiler chickens, while phytase additives may enhance calcium and phosphorus metabolism, offering potential benefits for optimizing nutritional strategies in the poultry industry.
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