|
چکیده
|
Background: Asthenozoospermia reduces sperm motility, leading to infertility. Sperm cryopreservation is an important technique in assisted reproductive technology which often causes significant damage to sperm quality. Adding antioxidants to the cryopreservation medium may mitigate these adverse effects. On the other hand, Theobromine (TB) is a plant alkaloid from the methylxanthine group, and is widely used for its antioxidant properties. Objective: This Experimental study aimed to assess the effect of TB supplementation on sperm biochemical parameters in asthenozoospermia men during cryopreservation. Materials and Methods: Semen samples were collected from 30 asthenospermic men in 1403 at Mehr Infertility Treatment Center, Rasht, Iran. Each sample was then divided into three groups (n1, n2, n3): n1: control (fresh), n 2 : freeze (treated with cryoprotectant), and n 3 : freeze + TB (treated with cryoprotectant and 10 mmol/L TB). 30 samples were examined in each group. In each sample, the level of sperm tumor necrosis factor alpha (TNF-α), malondialdehyde (MDA) and sperm antioxidant enzymes, including catalase, glutathione, and superoxide dismutase were analyzed using enzyme- linked immunosorbent. Results: The freeze group showed a significant reduction in the mean levels of sperm antioxidant enzymes, along with a significant increase in mean sperm TNF-α and MDA levels compared to the control group (p < 0.001). In contrast, the freeze + TB group showed a significant increase in the mean levels of sperm antioxidant enzymes and a significant decrease in mean sperm TNF-α and MDA levels compared to the freeze group (p < 0.001). Conclusion: This study found that cryopreservation harms sperm quality by lowering antioxidant enzyme levels and increasing TNF-α and MDA. TB supplementation improved antioxidant levels and reduced TNF-α and MDA, suggesting it may enhance sperm quality in asthenozoospermic men, potentially improving assisted reproductive outcomes.
|