Abstract Many flavonoids are active principles of medicinal plants, exhibit pharmacological effects and contribute to human health. Also they are taxonomically important for chemosystematic studies by the reason universal presence in vascular plants. In this study leaf, gall and bark flavonoids of nine populations of Quercus brantii L. varieties (Q. brantii var. belangeri and Q. brantii var. persica) are compared and reported for first time. Aqueous-ethanolic extracts of collected plant material from Lorestan Prvince aera, Iran were examined to practice flavonoid detection, isolation and identification by 2-dimensional paper chromatography (2-DPC), thin layer chromatography (TLC), UV spectroscopy and available references. Voucher specimens were prepared for reference as herbarium voucher. Results showed all of examined taxa have flavonoid sulphate, flavon C & C-/O glycosides and aglycons in their leaf, gall and bark. Apigenin, kaempferol, myricetin, naringenin, quercetin and rutin were nearly found in all of studied taxa parts. Studied samples leaf and bark had vitexin while their galls lacked.
