he purpose of the study was to evaluate the effectiveness of the canola oil on the some metabolites ostriches. In order to study the metabolic profile of ostriches in relation to diet, Six blue-neck male ostriches (Struthio camelus) were fed omega-3 resource (canola oil =3%) throughout a 60-day experiment. Blood samples were collected from ostriches on days 0 and 60 of the experiment to measure levels of total serum protein, albumin, total immunoglobulin, cholesterol, the activity of ASP, ALT, insulin and glucose. The results showed that from days 0 to 60 of the experiment, glucose and total protein levels increased significantly (P<0.05). whereas total immunoglobulins insulin, albumin, ALT and AST did not change.