most important environmental factor that reduces the salinity is already manufacturing plant. Osmolytes such as proline play a role in reducing the effects of salinity. Two varieties of cucumbers pre-treated with proline (0 and 20) mM and under different levels of salinity (0,50,100 and 150) mM in the culture in a petri was done. In this case, in the content of proline and total soluble carbohydrates and sodium and potassium as well as the elements and their ratio was increased with increasing stress. This will increase the plant's tolerance to salinity stress conditions. interaction of proline and salinity decreased in the total soluble carbohydrates, the ratio of sodium to potassium, sodium and increase the amount of potassium.