Background: Rheumatoid arthritis (RA) is a chronic autoimmune disorder primarily affecting the joints. Genetic factors play a significant role in the development and progression of RA. Several genetic polymorphisms have been associated with an increased risk of RA. Interleukin-10 (IL-10) is an important cytokine involved in regulating the immune response and inflammation. Polymorphisms in the IL-10 gene have been studied in relation to various diseases, including autoimmune disorders, infectious diseases, and cancer Objective: To investigate the association between, IL(10) genes polymorphisms and some other interleukins with Rheumatoid Arthritis in Kerbala women. Methods: In this case-control study, 70 RA female patients and 30 control were examined. The two groups were genotyped for the Two genes polymorphisms using tetra-primer ARMS–PCR and Allele Specific techniques. Body mass index (BMI), Anti-ccp, IL-6, IL-10, hs-CRP, total cholesterol, triglycerides, high density lipoprotein cholesterol (HDL-c), and low density lipoprotein cholesterol (LDL-c) were measured for all the study participants. The relation between the two genes polymorphisms, RA and the measured clinical parameters were statistically analyzed using appropriate tests. Results: Significant increases were recorded in the concentration of glucose, HbA1c, CK, TG, TC, VLDLc, LDLc, and atherogenic indices (Chol/HDLc, LDLc/HDLc, Ath.Co., and AIP) in the patients group as compared with the control group. While IL-10 showed a significant reduction in the patients' group compared with the controls. No statistically significant variation (all p>0.05) was found between RA patients and controls in the genes variants, according to statistical studies of genotypic and allelic rates for the examined SNPs. Under recessive and dominant models, the polymorphism frequencies of the control group and patient groups are significantly different (OR=2.354, p=0.045) under the recessive model. While the dominant variants showed no significant difference among groups (p>0.05). The frequency of the alleles A and C have a significant increase (OR=1.715, p=0.048) in patients compared with the control group. genotypes of SNPs of rs18000896 in patients and healthy control groups showed There is a highly significant reduction (p=0.002) in serum IL-10 of the AG patients group compared with both AA and GG groups. Conclusion: According to this study we can conclude that serum levels of some biochemical markers and IL-10 genes promoter polymorphisms can discriminate Rheumatoid Arthritis patients to detect the early stages of the disease and prevent its progression.
