Iran has different environmental conditions due to specific geographical. Therefore, exploring essential oil and flavonoids components among different medicinal plants in diffrenct area of the country may be caused to discoveries about valuable components. The present study aimed to study flavonoids profile and essential oils composition of four Lamiaceae species i.e. Stachys lavandulifolia, Teucrium polium, Thymus daenensis, and Ziziphora clinopodioides from Oshtorankoh located on Zagros Mountains, Iran. A chemical study using two dimentional paper chromatography (2-DPC) and thin-layer chromatography (TLC) showed that the four studied species contained flavone C and C-/O glucosides and flavonoid sulphates. The result of the present study revealed that T. polium had high levels of many flavonoids such as apigenin, genistein, isorhamnetin, kaempferol, quercetin, rutin, and vitexin compared to the other studied plants. According to the results of GC/MS analysis, S. lavandulifolia was rich in α-pinene (21.16 %) and 1,8-cineol (16.95 %), T. polium in α-pinene (12.21 %) and sabinene (9.55 %), T. daenensis in γ-Terpinene (36.98 %) and p-Cymene (26.87 %), and Z. clinopodioides in piperitone (16.84 %) and 1,8-cineol (16.11 %) components.
