In order to study the root flavonoids pattern of Convolvulus roots in 11 populations from four species (C. arvensis, C. commutatus, C. lineatus and C. pilosellaefolius) originated from Markazi Province, Iran, two dimensional paper chro-matography (2-DPC) and thin layer chromatography (TLC) were used. Results indicated that the roots contained flavonoid sulfates and flavones C and C-/O glycosides, apigenin, chrysin, genistein, kaempferol, luteolin, rhamnetin, rutin, and vitexin. hesperidin, isorhamnetin, morin, quercetin and tricine was not found in any of the taxa. Vitexin was the most found flavonoids in root. There was not any aglycone in the studied populations. All of populations had naringenin with the exception of CBB5, CBB2 and CBB6 populations. Myricetin was found in all of populations with the exception of CBB9 (C. commutatus). Populations of CBB19 (C. arvensis) had the most number of total flavonoids and C. lineatus and CBB9 (C. commutatus) had the least one.
