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Malek Soleimani mehranjani

Malek Soleimani mehranjani

Academic rank: Professor
ORCID: https://orcid.org/0000-0003-4822-2617
Education: PhD.
ScopusId: 6505623754
Faculty: Science
Address: Arak University
Phone:

Research

Title
Stereological study on the effect of silymarin on testis histology and daily sperm production in mice following treatment with cyclophosphamide
Type
Presentation
Keywords
Cyclophosphamide, Silymarin, Spermatogenesis, stereology.
Year
2023
Researchers Zahra Shaker Kordadeh ، Malek Soleimani mehranjani ، sepideh ahmadi

Abstract

Background: Cyclophosphamide is used in cancer treatment, but it also disturbs the testis tissue and the process of spermatogenesis through increasing oxidative stress. Objective: This study aimed to investigate silymarin’s effect on testicular histological alterations, spermatogenesis indexes, and daily sperm production in adult male mice following cyclophosphamide treatment. Materials and Methods: 24 NMRI male mice (37 ± 2 gr) were divided into 4 groups (n = 6/each): control; cyclophosphamide (100 mg/kg bw/wk, i.p); silymarin (200 mg/kg bw/interval day, i.p) and cyclophosphamide (100 mg/kg) + silymarin (200 mg/kg). After 35 days of treatment, daily sperm production, Johnsen’s score, and spermatogenesis indexes were evaluated. Also, the testis tissue was studied using a stereological technique. Results: A significant decrease in the total volume of the testis (43.18 ± 4.10), the volume of seminiferous tubules (34.09 ± 2.84) and interstitial tissue (9.08 ± 1.65), germinal epithelial height (24.86 ± 3.10), length (0.77 ± 0.10) and diameter of seminiferous tubules (127.10 ± 2.94), the number of spermatogonia (3.58 ± 0.51), spermatocyte (11.57 ± 1.34), elongated spermatid (20.66 ± 2.31), round spermatid (19.25 ± 2.10), Sertoli (1.78 ± 0.64) and Leydig cells (1.80 ± 0.15), spermatogenesis indexes (SPI: 44.00 ± 2.19; TDI: 51.50 ± 3.78; SCI: 14.96 ± 2.92; MI: 0.86 ± 0.02), Johnsen’s score (5.08 ± 0.03), and the daily sperm production (9.61 ± 2.39) was observed in the cyclophosphamide group compared to the control group (p < 0.01). The mentioned parameters were significantly increased in the cyclophosphamide + silymarin group compared to the cyclophosphamide group (p < 0.01). These parameters did not significantly differ between the control and silymarin groups (p > 0.05). Conclusion: According to the findings of this study, silymarin as an antioxidant can reduce the negative effects of cyclophosphamide on testis tissue, spermatogenesis indexes, and daily sperm production.