2024 : 5 : 23
Mahdi Khodaei Motlagh

Mahdi Khodaei Motlagh

Academic rank: Professor
ORCID: https://orcid.org/0000-0002-1281-7152
Education: PhD.
ScopusId: 41861677000
Faculty: Agriculture and Environment


The effect of in vitro Solanum melongena extract added to the semen extender on the progressive sperm motility after freezing in ram
extract, cryopreservation, spermatozoa, ram, progressive motility
Researchers hamidreza khodadadi ، Mahdi Khodaei Motlagh ، Mehdi Kazemi bonchenari ، Hamid Reza Momeni


This study aimed of to investigate the effect of in vitro addition t extract of Solanum melongenaon freeze and post thawing spermatozoa quality of ram (Farahani ram). In this study five healthy rams from idea reproduction body were used. Each sample of ram pooled together with other samples and then diluted at 37℃ with extender containing (control, 2, 4, 6 and 8 percent of Solanum melongena extract) and analyzed progressive sperm after freezing. Whereas, Each sample cooled and balanced at 5℃ in 0/5 straw, then estimated semen parameter (progressive sperm) after thawing straw in water 37℃. In conclusion, our result indicated that supplement 2 and 4 percent of eggplant peel extract to extender containing of Tris-yolk with 3.786 g Tris, 2.172 g citric acid and 1 g fructose in 100 ml distilled water. The diluent was supplemented with 5.0% (v/v) glycerol 15.0% egg yolk, penicillin (100,000), significantly improved progressive sperm post thawing compared to control group (p<0/01). In this study, levels of control (0), 2 and 4 percent of eggplant peel extract had equal significantly (p≤0/01). However, the supplement containing of 6 and 8 percent eggplant peel extract had a deleterious effect on progressive sperm motility spermatozoa after freezing. Materials and methods This experiment was carried at experimental station of Arak university. Also Extender of this study provided by a company (Merk Germany). Collection of semen was from four Farahani ram, after collection, semen samples immediately passed on to laboratory for analysis. Optimal progressive sperm motility was up 75 percent and under 10 percent progressive motility was not optimal for this analysis. In this experiment for each treatment used five repeats. Results In this assessment dilution containing about 6 and 8 percent eggplant peel extract didn’t expos significantly and gradually decrease progressive sperm motility, but levels of control (0), 2 and 4 percent extender containing explant peel extract had same