Thyme (Thymus daenensis subsp. Daenensis) is a perennial dwarf shrub growing 6–30 cm in height, belonging to Lamiaceae family [1]. Fourteen Thymus species grow in Iran , they are used as a medicinal herb and spice mainly due to the volatile constituents of plant. Thymus daenensis is an endemic species to Iran. The main volatile components of Thymus genus are thymol, arvacrol, p- cymene, g-terpinene, borneol, 1,8-cineol, terpinen-4-ol, a-terpineol and b-caryophyllene [2]. Most of the medicinal plants have some problems in seed germination and stand establishment in the field. Since germination and seedling establishment are critical stagesin the plant life cycle, offering the solutions for improvement of seed germination and seedling establishment willhelp to better performance in cultivation of medicinal plants [3]. In this research, the influence of different seed bed (coconut fiber, perlite, sand, soil, disinfection fertilizer and sand, fertilizer on morphological traitsincluding root length, lateral shoot number, shoot lenght, germination percentage and tolerance of plant to transplanting of thymus were evaluated under greenhouse conditions in a factorial experiment based on completely randomized design with four replications.Our findings revealed that the heighest shoot lenght were 11cm, and germination percentage 94% were obtained from (soil+sand+coconutfiber+perlite+fertilizer,2:2:2:1) seed beds. The most lateral shoot and the the lowest germination percentage 91% was due to (soil+sand+fertilizer, 1:1:1) seed beds. Also, the most root length 14cm was found in (coconut fiber+perlite,1:1) besides the most drying of seedlings. But (soil+sand+fertilizer, 1:1:1) indicated the best seed bed for transplanting.
