In this research for antibacterial membranes preparation, Pseudomonas bacteria were synthesized and used for antibacterial activity by weight percentage 0.25, 0.5 and 1 in the mixtures. These mixtures were then immersed in a non-solvent bath (distilled water) after being pulled by a film applicator. DLS nanoparticle analyzer was used to detect nanoparticles and particles size. Also to identify silver nanoparticles in membranes structure, X-ray fluorescence (XRF) spectroscopy was used. To evaluate the antibacterial properties of the membranes Disk test was used using Escherichia coli (E. coli) as a gram-negative bacterium and Staphylococcus aureus (S. aureus) as a gram-positive bacterium. To test the performance of the fabricated membranes, the flow rate was measured and the rejection test was performed. After determining the presence of nanoparticles in the membrane structure, the DLS nanoparticle analyzer determined the size of the synthesized nanoparticles under optimal conditions between 0.46 and 0.54 nm. The permeability and antibacterial properties of membranes against gram-positive and gram-negative bacteria increased with increasing silver nanoparticles.
