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Ali Khadivi

Ali Khadivi

Academic rank: Professor
ORCID: https://orcid.org/0000-0001-6354-445X
Education: PhD.
ScopusId: 43661256800
HIndex:
Faculty: Agriculture and Environment
Address: Arak University
Phone: 086-32623022

Research

Title
Population structure and genotypic variation of Crataegus pontica inferred by molecular markers
Type
JournalPaper
Keywords
Forest genetics Genetic diversity Crataegus Molecular polymorphism Germplasm conservation Population variability
Year
2015
Journal Gene
DOI
Researchers Mohammad Shafie Rahmani ، Naghi Shabanian ، Ali Khadivi ، کیت Woeste ، Hedieh Badakhshan ، Leila Alikhani

Abstract

Information about the natural patterns of genetic variability and their evolutionary bases are of fundamental practical importance for sustainable forest management and conservation. In the present study, the genetic diversity of 164 individuals from fourteen natural populations of Crataegus pontica K.Koch was assessed for the first time using three genome-based molecular techniques; inter-retrotransposon amplified polymorphism (IRAP); inter-simple sequence repeats (ISSR) and start codon targeted (SCoT) polymorphism. IRAP, ISSR and SCoT analyses yielded 126, 254 and 199 scorable amplified bands, respectively, of which 90.48, 93.37 and 83.78% were polymorphic. ISSR revealed efficiency over IRAP and SCoT due to high effective multiplex ratio, marker index and resolving power. The dendrograms based on the markers used and combined data divided individuals into three major clusters. The correlation between the coefficient matrices for the IRAP, ISSR and SCoT data was significant. A higher level of genetic variation was observed within populations than among populations based on the markers used. The lower divergence levels depicted among the studied populations could be seen as evidence of gene flow. The promotion of gene exchange will be very beneficial to conserve and utilize the enormous genetic variability.