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Seyed Mohammadali Shariatzadeh

Seyed Mohammadali Shariatzadeh

Academic rank: Professor
ORCID: https://orcid.org/0000-0002-2395-8057
Education: PhD.
ScopusId: 15133044400
Faculty: Science
Address: Arak University


The Effect of Sildenafil Citrate and Alpha Lipoic Acid Co- treatment on the Sperm Parameters during Cryopreserva- tion in the Asthenozoospermic Infertile Men
Cryopreservation, Sperm parameters, Asthenozoo- spermia, Sildenafil Citrate, Alpha Lipoic Acid
Researchers Ronak Kohzadi ، Malek Soleimani mehranjani ، Seyed Mohammadali Shariatzadeh ، Ebrahim Cheraghi


Background: Asthenozoospermia is a kind of male infertility that reduces sperm motility. Sperm cryopreservation is used in assisted reproductive technology (ART), gamete preserva- tion before radiotherapy or chemotherapy, and sperm banking. Freezing process causes oxidative stress on sperms which is the main reason of sperm disruption. The sperm cells protection against oxidative reactions during cryopreservation process is done by antioxidant and amino acids agents. In this study we evaluated the effect of Sildenafil Citrate (SC) and Alpha Lipoic Acid (ALA) Co-treatment, in preventing sperm damage during Cryopreservation in the asthenozoospermic patients. Materials and Methods: 30 semen samples were collected from the asthenozoospermic patients attended the Andrology unit of the Qom Infertility Treatment Center. Each semen sam- ple was divided into 4 equal aliquots: Control (were treated with cryo-protectant alone), SC (were treated with cryo-protectant + 0.67µM SC), ALA (were treated with cryo-protectant + 0.5mM ALA), SC+ALA. In each sample, sperm motility according to WHO criteria, sperm viability with eosin-nigrosin assay and sperm morphology with Diff-Quick assay were assessed. Results: Our result showed that sperm parameters were sig- nificantly higher in ALA group compared to control, SC and SC+ALA groups (p<0.05). And SC+ALA group compared to SC group had more positive impact. Conclusion: All ALA, SC and SC+ALA groups improved se- men sample parameters after cryopreservation. But ALA group performed several percent better than the other groups.