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Mahdi Khodaei Motlagh

Mahdi Khodaei Motlagh

Academic rank: Professor
ORCID: https://orcid.org/0000-0002-1281-7152
Education: PhD.
ScopusId: 41861677000
Faculty: Agriculture and Environment
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Research

Title
Tumor necrosis factor receptor type-1 is not expressed on granulosa cells of pre-ovulatory follicles in sheep
Type
JournalPaper
Keywords
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Year
2013
Journal Research Opinions in Animal and Veterinary Sciences
DOI
Researchers Mohammad Yahyaei ، Mohammad Khourvash ، Hamid R Rahmani ، Mahdi Khodaei Motlagh ، Hamid R Mohammadnia

Abstract

This study was conducted to examine the expression of tumor necrosis factor receptor type1 (TNFR-I) on granulosa cells of pre-ovulatory follicles in sheep. In all ewes (n=15), estrus was synchronized with the Controlled Internal Drug Release CIDR (12 day). The granulosa cells were collected from pre-ovulatory follicle 48 hours after CIDR withdrawal. Expression of mRNA was investigated using a reverse transcriptase-polymerase chain reaction (RT-PCR). Total RNA was extracted from cells and then reverse-transcribed to synthesize cDNA and PCR-amplified using specific oligonucleotide primers. Result of present study indicates TNFR-1 was not expressed at the mRNA level. In general, this finding provides further insight into the proposed physiologic roles of TNFα in ovarian function.