1403/01/26
مهدی خدایی مطلق

مهدی خدایی مطلق

مرتبه علمی: استاد
ارکید: https://orcid.org/0000-0002-1281-7152
تحصیلات: دکترای تخصصی
اسکاپوس: 41861677000
دانشکده: دانشکده کشاورزی و محیط زیست
نشانی: دانشگاه اراک
تلفن:

مشخصات پژوهش

عنوان
اثر عصاره رزماری به عنوان یک عامل آنتی اکسیدان در محیط انجماد بر پایه لسیتین سویا بر کیفیت اسپرم قوچ پس از فرایند انجماد- ذوب
نوع پژوهش
طرح پژوهشی خاتمه‌یافته
کلیدواژه‌ها
عصاره رزماری آنتی اکسیدان اسپرم انجماد
سال 1393
پژوهشگران مهدی خدایی مطلق

چکیده

The aim of current study was to evaluate effect of rosemary aqueous extract on post-thawed ram sperm quality in a soybean lecithin-based (SL) extender. Ram semen samples were obtained, extended with SL extender and supplemented with 0% (SL-R0), 2% (SL-R2), 4% (SL-R4), 6% (SL-R6), and 8% (SL-R8) rosemary aqueous extract. Following equilibration, the straws were frozen, and then plunged into the liquid nitro-gen. After thawing, sperm motility and velocity parameters, plasma membrane functionality, viability, acrosomal and capacitation status were evaluated. Membrane lipid peroxidation was also analyzed through the malondialdehyde (MDA) concentration. Our results showed that SL-R4 and SL-R6 groups resulted in higher ( p < 0.05) percentages of total motility, progressive motility, and plasma membrane functionality, as compared with other groups. Highest ( p < 0.05) viable and lowest (p < 0.05) dead spermatozoa were observed in SL-R6 group compared to the other groups. The acrosomal and capacitation status were not affected ( p > 0.05) by different levels of rosemary aqueous extract. Lower ( p < 0.05) MDA concentration has been observed in SL-R4 and SL-R6 groups. The results of this study demonstrate that supplementation of SL extender with rosemary aqueous extract influences post-thawed ram spermquality in a dose dependent manner.