1403/01/31
مهدی خدایی مطلق

مهدی خدایی مطلق

مرتبه علمی: استاد
ارکید: https://orcid.org/0000-0002-1281-7152
تحصیلات: دکترای تخصصی
اسکاپوس: 41861677000
دانشکده: دانشکده کشاورزی و محیط زیست
نشانی: دانشگاه اراک
تلفن:

مشخصات پژوهش

عنوان
بررسی فولیکول های آنترال تخمدانی برای تعیین غلظت آلفا-لینولنیک موثر بر توانایی تکوین برون تنی اووسیت های بز
نوع پژوهش
مقاله چاپ‌شده
کلیدواژه‌ها
آلفا الینولنیک اووسیت بلاستوسیت بلوغ برون تنی و مایع فولیکولی
سال 1393
مجله پژوهش هاي علوم دامي ايران
شناسه DOI
پژوهشگران آرش وشکینی ، عبدالله محمدی سنگ چشمه ، علی اکبر خادم ، علی اسدی الموتی ، مهدی خدایی مطلق

چکیده

Besides their role in making energy, polyunsaturated fatty acids can affect on ovarian ollicles and corpus luteum by providing the precursors required for the synthesis of gnaling molecules such as steroids and prostaglandins. The effects of α-linolenic acid ALA) on developmental competence of oocytes in goats were evaluated in this study. nitially, ALA content of follicular fluid of small and large follicles was determined using C–mass chromatography. Our results indicated that the ALA concentration was in a range 64.6 to 100.6 μM for small and large follicles, respectively. Then, based on preliminary sults, goat oocytes were matured in presence of 0 (Control), 10 (ALA-10), 50 (ALA-50), 100 (ALA-100)and 200 (ALA-200)μM of ALA. Twenty four hours after in vitro aturation (IVM), oocytes in each group were evaluated for their cumulus cell expansion nd also for their maturation rate. Furthermore, matured oocytes were treated for parthenogenetic activation and the cleavage and blastocyst rates of control and ALA-treated oup were recorded at day 3 and 8 post-activation. Data from each stage were recorded nd analyzed via Compare Means Test and/or chi-square analysis using SAS software. upplementation of maturation media with different concentration of ALA had no effect on umulus cell expansion except in the highest concentration (ALA-200)that decreased the umulus cell expansion (P˂0.05). Our findings indicated that maturation rate was higher P˂0.05)in ALA-50 group as compared with the control group (68.1% vs. 58.2%). Moreover, supplementation of maturation medium with 50 μM ALA improved the cleavage te (65.2% vs. 52.8%) and blastocyst rate (25.1% vs. 16.7%)as compared with the control oup (P˂0.05). Collectively, our results showed that treatment of maturation medium with optimum concentration of ALA had a beneficial effect on oocyte maturation by increasing he maturation rate and this in turn, can stimulate the embryonic development. 1- M.Sc. Student, Pardis Aboreyhan of T