1403/01/31
مهدی خدایی مطلق

مهدی خدایی مطلق

مرتبه علمی: استاد
ارکید: https://orcid.org/0000-0002-1281-7152
تحصیلات: دکترای تخصصی
اسکاپوس: 41861677000
دانشکده: دانشکده کشاورزی و محیط زیست
نشانی: دانشگاه اراک
تلفن:

مشخصات پژوهش

عنوان
Antioxidant effect of rosemary (Rosmarinus officinalis L.) extractin soybean lecithin-based semen extender following freeze–thawing process of ram sperm
نوع پژوهش
مقاله چاپ‌شده
کلیدواژه‌ها
Antioxidant effect Rosemary Ram semen Soybean lecithin Sperm freeze–thaw process
سال 1393
مجله Cryobiology
شناسه DOI
پژوهشگران مهدی خدایی مطلق ، محسن شرفی ، مهدی ژندی ، عبدالله محمدی سنگ چشمه ، ملک شاکری ، مسعود سلیمانی ، سعید زین الدینی

چکیده

The aim of current study was to evaluate effect of rosemary aqueous extract on post-thawed ram sperm quality in a soybean lecithin-based (SL) extender. Ram semen samples were obtained, extended with SL extender and supplemented with 0% (SL-R0), 2% (SL-R2), 4% (SL-R4), 6% (SL-R6), and 8% (SL-R8) rosemary aqueous extract. Following equilibration, the straws were frozen, and then plunged into the liquid nitrogen. After thawing, sperm motility and velocity parameters, plasma membrane functionality, viability, acrosomal and capacitation status were evaluated. Membrane lipid peroxidation was also analyzed through the malondialdehyde (MDA) concentration. Our results showed that SL-R4 and SL-R6 groups resulted in higher (p < 0.05) percentages of total motility, progressive motility, and plasma membrane functionality, as compared with other groups. Highest (p < 0.05) viable and lowest (p < 0.05) dead spermatozoa were observed in SL-R6 group compared to the other groups. The acrosomal and capacitation status were not affected (p > 0.05) by different levels of rosemary aqueous extract. Lower (p < 0.05) MDA concentration has been observed in SL-R4 and SL-R6 groups. The results of this study demonstrate that supplementation of SL extender with rosemary aqueous extract influences post-thawed ram sperm quality in a dose dependent manner.  2014 Elsevier Inc. All rights reserved.