In this paper, the synthesis of vanadium oxide by hydrothermal method and in the presence of Template molecules, which are different carboxylic acids ( malonic acid, succinic acid, adipic acid and glutaric acid ) of chain length, have been reported. The effect of the type of carboxylic acid on the structure and morphology of the catalysts were characterized by X-ray diffraction (XRD), scanning electron microscope (SEM) and FT-IR analysis. The results revealed that the morphologies of the vanadium pentoxide Nano crystals could be controlled by template molecules. This facile method to fabricate V2O5 Nano particle with well-defined morphologies would be of great significance to design other complicated micro-architectures with advanced functions.