Parrotia persica C.A. Meyer is a relict endemic species of Hamamelidaceae. It had a wide range of distribution before the Pleistocene glaciations, but is currently restricted to the Hyrcanian forests of Iran. These tress pose biological activities against some pathological organisms and have a great potential to use them in parks and streets. In the current study, we investigated infraspecific genetic diversity and structure in 10 populations of P. persica. The nucleic genomes were extracted using the CTAB modified protocol. Then, we used 10 SCoT molecular primers for PCR amplification. The following software were employed for molecular analyses: POPGENE ver. 1.31, GenAlex ver. 6.4, Population Analysis with Reticulate Trees (PopART) ver. 1.7, Geno–Dive ver. 2 and STRUCTURE ver.2.3.4. We determined a significant genetic diversity using the AMOVA test that its great part assigned to among populations. These findings were supported by almost all of the evaluated genetic diversity and polymorphism parameters. Moreover, a high level of genetic differentiation (Gst) and total genetic variation (Ht) were recorded among the populations, which were in accordance with a low rate of gene flow and reticulation tree of the evaluated populations. We registered a high level of genetic polymorphism in some populations, which resulted from a large amount of genetic mutation among their individuals. Mantel