Investigation about the activity pattern of the Jerboas in different habitat types including scares semi-desert steppe habitat types with low and scares vegetation in the central parts and mountainous steppes in the north-western parts of Iran confirmed that habitat structure can strongly affect nocturnal activity behaviour of small mammals with unique bipedal movement. In both habitat types we recorded more encounter rate by the individuals in the first and last weeks of the lunar nights but the pattern of habitat selection showed significant differences. Statistical analysis for investigating about the mean difference of used plots vegetation percent cover and habitat types in both low and high moon periods showed that habitat structural feature can significantly affect the species habitat usage pattern. Bipedal locomotion of the species need specific antipredator habitat selection mechanism that is differ from other small mammals.