Introduction: Docetaxel with the trade name Taxotere is one of the most important taxane compounds for cancer treatment in recent years, which causes disorders in the male reproductive system. In this study, the antioxidant effect of folic acid on the adverse effects of docetaxel on testicular tissue was investigated. Methods and Materials: In this experimental experiment, 24 adult male NMRI mice (35-40g) were divided into 4 groups: control, docetaxel (5 mg/kg, once every week), folic acid (1 mg/kg, daily), docetaxel+folic acid and intraperitoneal injection was done for 28 days. After the last treatment, the mice were anesthetized and the left testis were used to estimate the total volume of the testis, the volume, length and height of the epithelium of the seminiferous tubules as well as the number of germ and sertoli cells. The results were statistically analyzed by One-Way ANOVA and Tukey's test and considered significant at the p<0.05 level. Results: A significant decrease was observed in weight and volume of testis, the volume, length and height of seminiferous tubules and the number of germ cells in the docetaxel group compared to the control group (p<0.05). Testosterone levels also decreased significantly in the docetaxel group compared to the control group (p<0.05), Simultaneous treatment of folic acid with docetaxel significantly improved the mentioned parameters compared to the docetaxel group. Conclusion: The results showed that folic acid as a potent antioxidant can prevent testis tissue damage induced by docetaxel.
