Microorganisms, that are part of the organic matter in the wastewater, can affect human health, because they are present in fecal material. Some chemical and physical agents, such as chlorine, ultraviolet light, reverse osmosis, and silver catalyst are well developed [1,2]. The bactericide activity of Copper ions has been known for a long time. Copper is an essential mineral perceived by the public today as being of "exceptional biologic and public health importance", especially regarding prenatal and postnatal development [3]. In this work Cu/4A Zeolite compound was prepared and characterized by using FT-IR, XRD, SEM techniques. In Fig.1 the FT-IR spectrum of Cu/4A Zeolite indicate an intense band about ca.1011 cm-1 attributable to the asymmetric stretching of Al–O–Si chain of zeolite. The symmetric stretching and bending frequency bands of Al–O–Si framework of zeolite appear at ca.696 and 453 cm-1, respectively. The result in Fig.2 showed that the synthesized Cu/4A Zeolite powder were particle Shape with the diameter of 12-18 nm and were made. Then their antibacterial properties against different bacteria (gram-positive and gram negative bacteria) was investigated. Figure 1. The FT-IR of Cu/4A Zeolite Figure 2. The SEM image of Cu/4A Zeolite