In order to study the lipid profile of ostriches in relation to diet, 6 blue-neck male ostriches (Struthio camelus) were used and fed with 3% canola oil supplement on diets adlibitum consisting. In the morning, after about 12 h of fasting, blood was collected from the wing vein on days 0 and 60. Blood samples were collected from ostriches to measure levels of the following lipid parameters include high-density lipoprotein (HDL) cholesterol, low-density lipoprotein (LDL) cholesterol, very low-density lipoprotein (VLDL), triglyceride, total cholesterol were determined. Results showed that from days 0 to 60, LDL and total cholesterol levels increased significantly, whereas HDL, VLDL and triglyceride didn't change.