The effect of geometry and material of the Micro/Nano particle on contact mechanic for manipulation was studied in this work based on atomic force microscopy. Hertz contact model simulation for EpH biological micro particle with spherical, cylindrical, and circular crowned roller shape was used to investigate the effect of geometry on contact simulation process in manipulation. Then, to validate the simulation results, they were compared to experimental ones. The results can be interpreted from two perspectives, first from the perspective of types of nanoparticles and second from the perspective of types of theories.To investigate the effect of the material of micro/nano particle in contact mechanic simulation process, spherical contact simulations of two EpH and C3T3 cells were compared with each other. EpH cells simulations with different geometries showed that the cylindrical shape estimation did not provide accurate response due to longer contact length. However, spherical and circular crowned roller estimations which had 19.6% and 15.6% difference from experimental results, respectively, had relatively accurate response. Therefore, selection of circular crowned roller geometry will produce more logical response. Also, the comparison of spherical contact simulation of EpH and C3T3 cells showed that C3T3 cell shows more indention depth under the same applied force.
