Flavonoids are as one set of the polyphenolic compounds among secondary metabolites in different organs of plants that possess a wide range of biological activities). More than 4000 varieties of flavonoids have been identified in different higher and lower plant species. Many flavonoids are active principles of medicinal plants and exhibit pharmacological effects. Numerous epidemiological studies confirm significant relationship between the high dietary intake of flavonoids and the reduction of cardiovascular and carcinogenic risk. They are also beneficial for the plant itself as physiological active compounds, as stress protecting agents, as attractants or as feeding deterrents, and, in general, by their significant role in plant resistance. Also these compounds serve essential functions in plant reproduction by recruiting pollinators and seed disperses. They are also responsible for the beautiful display of fall color in many plant species, which has recently been suggested to protect leaf cells from photo-oxidative damage, thereby enhancing the efficiency of nutrient retrieval during senescence. The aim of this study was to compare the leaf flavonoids profiles of some Iranian Angiosperm species from Leguminosae, Polygonaceae, Euphorbiaceae, Resedaceae and Cyperaceae using chromatographical methods. Studies of leaf flavonoids showed some phytochemical characters such as total number of flavonoids, flavonoid group such as aglycone, flavones C- and C-/O glycoside and flavonoid sulphate and kind of flavonoids such as kaempherol, quercetin, myricetin are valuable for chemotaxonomy and their usage.