In this study, root, leaf and fruit flavonoids of Tribulus terrestris L. are compared. Aqueous-ethanolic extracts of collected plant material were examined to practice flavonoid detection, isolation and identification by 2-dimensional paper chromatography, thin layer chromatography and available references. Voucher specimen was prepared for reference as herbarium voucher. Results showed chrysin was just identified in fruit while root and leaf had not any chrysin. Also flavone C and C-/O-glycosides were not found in leaf and fruit whereas root of the species had flavonoid sulphates and aglycones in addition to flavone C and C-/O-glycosides.
