Abstract In this research, fruit flavonoids characters of some collected Euphorbia species (subgenera Esula and Chamaesyce) from Markazi province, Iran area were studied using 2-Dimentional Paper Chromatography (2-DPC), Thin Layer Chromatography (TLC) and available standard flavonoids. Voucher specimens were prepared for reference as herbarium voucher. Our studies showed that all of studied Euphorbia species had flavonoids considerably. Flavonoid sulphates and flavone C- and C-/O-glucosides existed in fruit of studied taxa, while dihyro flavonol 3-O-monoglycosides just were found in E. falcata. Kaempferol were detected in all of studied species in contrast to rhamnetin which was found only in E. ozyridiforma. Concenteration and variety of flavonoid componds in studied taxa of subgenera Esula were more than subgenera Chamaesyce. Some flavonoid compounds including myricetin, naringenin and rhamnetin were not detected in Euphorbia studied species of subgenera Chamaesyce.
