Cluster bean guar or (Cyamopsis tetragonoloba L.) a valuable and multipurpose legume, due to its galactomannan and the seeds high in protein, is widely used in different industries. A field study was carried out using factorial experiment in a randomized complete block design with three replications to evaluate the influence of different sowing times (May 15, June 22, and July 27) on performance (seed yield, harvest index, biological yield, etc.), qualitative attributes (galactomannan, protein, carbohydrate, etc.), metabolic traits (lipid, pHenol, fiber, etc.) and yield components (branch/plant, pod/plant and plant height) of 20 guar genotypes (RGC-1066, BR-99, Sarbaz, HG-867, RGC-936, S-5488, S-5490, Dalgan, Iranshahr, Pishen, RGC-1038, RGC-1017, S-5498, RGC-1031, Saravan, S-5499, RGC-986, RGC-1003, HG-884 and HG-563) from three different origins (India, Iran and Pakistan). According to the ANOVA results, plant height, harvest index, the number of pod/plant, total chlorophyll, carbohydrate rate, gum content, and gum yield were significantly changed (p < 0.01) by sowing dates. Guar traits such as plant height, the length of crop cycle, number of pod/plant, harvest index, and protein yield were significantly (p < 0.01) impacted by a two-factor interaction (planting time × genotypes). Based on the results, RGC-1066 and Dalgan showed the maximum and minimum amount of seed yield, respectively. However, the maximum and minimum amount of protein was obtained from RGC-1003 and RGC-1038, respectively. July × HG-884 and July × HG-563 interactions showed the maximum amount of protein yield. The number of branch/plant, pod/plant, harvest index, gum content, and gum yield showed a positive significant correlation with seed yield. Regarding galactomannan and protein contents, HG-884 and RGC-1066 are suitable for gum extraction and guar meal, whereas HG-563 and RGC-1003 due to the high protein content are suitable for meal. RGC-1066 and Iranshahr exhibited the maximum amount of c
