Purpose: Currently, the most useful questionnaire for determining the chronotype is the Munich ChronoType Questionnaire (MCTQ). It determines chronotype based on sleep-wake behavior on workdays and work-free days and uses the mid-sleep time on free days (MSF), corrected for accumulated sleep debt over the work week to classify chronotype (MSFsc). Our study aimed at validating Persian version of the MCTQ. Methods: Participants completed the Persian version of the MCTQ, reduced Morningness-Eveningness Questionnaire (rMEQ), Epworth sleepiness scale (ESS), and demographic characteristics. Results: The study sample was comprised of 250 Iranian men and women (mean age 34 years, age range 30 to 58 years, 113 men). Results showed that there is a significant negative relationship between the components of MCTQ and rMEQ, and the strongest relationship related to the MSF index. The results also showed that MCTQ parameters were not significantly related to the ESS scale. Cut-off values of MSFsc for chronotype categories were determined by inter-quartile range (Q25% = 3.44, Q50% = 4.68, and Q75% = 5.75). Conclusions: Like the MCTQ in other languages, the Persian version of the MCTQ is a reliable tool for assessing chronotype.