zinc oxide nanoparticles have been synthesized using nigella sativa L. seed extract. Nigella sativa L. is an annual herbaceous plant belonging to the Ranunculaceae family. Concentration of plant extract plays a vital role in the synthesis of nanoparticles zinc oxide. Nanostructures were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM). This experiment was conducted in Arak University in an experiment based on randomized complete block design with four replications. Each replication consisted of one fertilizer levels including 2 per thousand of Zn-nanoparticles in one stages of growth (8 or 12 leaves). During the experiment, the height of plant, number of branches was investigated. This study showed that using spraying had significant differences in the factors like plant height number of branches. Also, using all microelement treatments had significant effects to the level of 1%. In case of using spraying treatments, the best results for number of branches and height were related to 2.perthousand of Zn- nanoparticles and the least were related to control. This formulation can be used for increasing yield, enhancing the products and removing food deficiencies.
