This study was conducted with the aim of to evaluating the effect of foliar spray different concentrations of spherical nano-carbon on the physiological and metabolic traits of feverfew (Tanacetum parthenium L.). The active ingredient of this plant is Parthenolide, which has anti-migraine properties. Experiments were conducted in a completely randomized design with five treatments (0, 125, 250, 500 and 1000 mg / l) with three replications at the Faculty of Agriculture and Natural Resources, University of Arak, in 1395. The Investigated traits in this study are photosynthetic pigment content (chlorophyll a and b and carotenoid), essential oil percentage, and parethenolide content of leaf and flower extract. The spherical nano-carbon used in this study was prepared with 95% purity and 20 to 40 nm in size from the pioneering IRANian Nanotechnology Company (Mashhad). The results of the experiment showed that spherical nano-carbon concentrations in chlorophyll a and b content, essential oil percentage and partinolide content were statistically significant (P0.01) and carotenoid content (P0.05). The content of chlorophyll a (56.5%) chlorophyll b (59.6%) in 1000 mg / l concentration of nano-carbon spherical was higher than control. Additionally, in the concentration of 250 mg / l, the spherical nano-carbon increased the content of carotenoids by 34.7% than the control. Parthenolide content increased 100% in all concentrations and the percentage of essential oils affected by these treatments was decreasing.