2024 : 11 : 5
Malek Soleimani mehranjani

Malek Soleimani mehranjani

Academic rank: Professor
ORCID: https://orcid.org/0000-0003-4822-2617
Education: PhD.
ScopusId: 6505623754
HIndex:
Faculty: Science
Address: Arak University
Phone:

Research

Title
The effect of vitamin C on the recovery of activity and survival of autografted ovaries through inhibition of oxidation and inflammation
Type
JournalPaper
Keywords
Ovary autografting, Vitamin C, Apoptosis, Inflammation, Oxidative stress, Mice
Year
2024
Journal tissue and cell
DOI
Researchers Maedeh Talesh Sasani ، Monireh Mahmoodi ، Malek Soleimani mehranjani

Abstract

Ovarian tissue autografting is a valuable clinical option to help restore fertility in women with cancer. However, many follicles are lost due to ischemia-reperfusion (IR) injury, which depletes follicles after grafting. We aimed to investigate the effect of vitamin C, an antioxidant with anti-apoptotic and anti-inflammatory properties, on improving the structure and function of autografted ovaries in mice. Thirty-six female NMRI mice (4–5 weeks old) were divided into three groups of 12: control (no grafting), autograft + vitamin C (50 mg/kg/day intra- peritoneally), and autograft + saline (100 µl/day/animal, intraperitoneally). After the ovarian autografting and before the start of the experiment, each group was further divided into 7-day and 28-day subgroups. Seven days after ovary autografting, serum levels of malondialdehyde (MDA), total antioxidant capacity (TAC), and in- flammatory factors were measured. On day 28, ovarian histology, DNA fragmentation, and estradiol and pro- gesterone levels were assessed. Results were analyzed using one-way ANOVA and Tukey’s test, with significance set at p<0.05. In the autograft + vitamin C group, there were significant increases in the mean total volume of the ovary, cortex (p<0.05), medulla, number of follicles, and levels of IL-10, progesterone, estradiol, and TAC (p<0.001), compared to the autograft group. Conversely, the rate of apoptosis and serum levels of MDA, IL-6, and TNF- α were notably reduced in the autograft + vitamin C group (p<0.001). These results suggest that vitamin C can significantly enhance the recovery of autografted ovaries through its antioxidant, anti- inflammatory, and anti-apoptotic effects.