Background: One of the challenges following ovarian trans- plantation is ischemia/reperfusion injury that leads to massive depletion of the follicular reservoir and tissue damage. There- fore, it is necessary to find ways to reduce hypoxia particularly during the early post-transplant stages. Platelet lysate (PL) as a rich source of growth factors can be used to improve transplant survival through reducing oxidative stress and inflammation. Materials and Methods: In this experimental study, the Naval Medical Research Institute (NMRI) mice (4–5 weeks old) were divided into three groups: control, autograft and autograft+PL (5ml/kg at the graft sites). Seven days after ovary autograft- ing, blood samples were collected and serum levels of Malon- dialdehyde (MDA), total antioxidant capacity, tumor necrosis factor alpha (TNF-α), interleukin (IL)-6 and IL-10 were meas- ured. Data were analyzed using one-way analysis of variance (ANOVA) and Tukey test, and the means were considered sig- nificantly different at P<0.05. Results: The serum concentrations of IL-6, TNF-α and MDA increased significantly in the autograft group compared to the control group whereas these parameters reduced significantly in the autograft+PL group. Total antioxidant capacity and the serum level of IL-10 also reduced significantly in the autograft group when compared to the control while it significantly in- creased in the autograft+PL group. Conclusion: According to our results, PL can act as a substrate to prepare the ovarian tissue grafting site due to its antioxidant and anti-inflammation properties.
