tioxidant properties that can be ef- fective in the treatment of infertility, cardiovascular diseases, human immunodeficiency virus infections, liver poisoning and metal poisoning. Since the effect of NAC on normal sperm cells has not been measured and it is necessary to understand the mechanism of its effect on sperm cells before considering its use in clinical conditions, our aim is to investigate the effect of NAC on motility, acrosome reaction and DNA integrity of normal sperm. Materials and Methods: Normal sperm samples of 30 fertile men were collected. Then, each sample was divided into three groups: fresh, control (untreated after 1 hour of incubation) and NAC (treated with 50 µM NAC antioxidant after 1 hour of in- cubation). The sperm mobility in each sample was measured with a light microscope. Also, acrosome reaction and DNA fragmentation were evaluated using FITC- Pisum sativum ag- glutinin and acridine orange staining, respectively. The data was analyzed using repeated measures analysis. Results: A significant increase in the mean sperm total and pro- gressive motility was observed in the NAC group compared to the fresh and control groups (P˂0.001). NAC was able to sig- nificantly reduce the nonprogressive motility, premature acro- somal reaction and sperm DNA fragmentation compared to the control group (P˂0.01). Conclusion: Our observations showed that NAC has positive effects on normal sperm and can improve progressive and total sperm motility, DNA integrity and acrosomal integrity.
