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Majid Mahdiyeh

Majid Mahdiyeh

Academic rank: Associate Professor
ORCID: https://orcid.org/0000-0001-5588-186X
Education: PhD.
ScopusId: 55748908200
HIndex:
Faculty: Science
Address: Arak University
Phone:

Research

Title
Production of Recombinant Human Interleukin-11 (IL-11) in Transgeni Tobacco (Nicotiana tabacum) Plants
Type
JournalPaper
Keywords
Cytokine, Recombinant human interleukin-11, Transgene expression, Transgenic plant
Year
2016
Journal Journal of Plant Biotechnology
DOI
Researchers abdol rahim sadeghi ، Majid Mahdiyeh ، ُSomayeh Sadeghi

Abstract

Interleukin-11 (IL-11) is a cytokine that plays a key regulatory role in the immune system. Recombinant human IL-11 (rhIL-11) exerts a preventative effect against apoptotic cell death and inhibits preadipocyte differentiation. IL-11 also is used to stimulate the bone marrow to produce platelets in order to prevent low platelets that may be caused by chemotherapy. Unfortunately, the high production cost of IL-11 associated. In this study, we investigated the feasibility of transgenic plants for the cost-effective production of rhIL-11. Production of rhIL-11 proteins in whole-plant expression system will be more economical when compared to the current E. coli based expression system. The human rhIL-11 gene was codon optimized to maximize plant host system expression. IL-11 expression vector under the control of a constitutive cauliflower mosaic virus 35S (CaMV 35S) promoter was introduced into tobacco by Agrobacteriummediated transformation. The 5′-leader sequence (called Ω) of tobacco mosaic virus (TMV) as a translational enhancer was added to construct. Transgenic tobacco plants expressing various levels of rhIL-11 protein were generated. Western blotting of the stably transformed lines demonstrated accumulation of the appropriately sized rhIL-11 protein in leaves. This research demonstrated the efficacy of using tobacco as an expression system for the production of rhIL-11.