There are many plain concrete arch bridges in Iran that have been used as railway bridges for more than seventy years. Owe to the fact that these bridges have not been designed seismically, and even may be loaded under high-speed trains, evaluation of fundamental frequencies of the bridges against earthquake and high-speed train vibrations is necessary for considering dynamics effects. To evaluate complex behavior of these bridges, results of field tests are useful. Since it is not possible to perform field tests for all arch bridges, these structures should be simulated correctly by computers for structural assessment. Several parameters are employed to describe the bridges, such as number of spans, length of spans, geometrical and material properties. In this study, results of field tests are used for modal analysis and adapted for 64 three dimensional finite element models with various physical parameters. Computer simulations show length of spans has important effect on fundamental frequencies of plain concrete arch bridge and modal deformations of bridges is in longitudinal and transverse directions. Also, these results demonstrate that fundamental frequencies of bridges decrease after increasing span length and number of spans. Plus, some relations based in the number of spans (n) and span length (l) are proposed for calculation of fundamental frequencies of plain concrete arch bridge.