This experiments was conducted to evaluate the effect of different levels of royal jelly (RJ) on some goat semen parameters within the freezing and thawing process. The semen was collected from four mature regular donor Mahabadi bucks, ejaculates were pooled and semen evaluated initially. After preparing the treatments with desired concentrations of royal jelly (0.5, 1, 1.5, and 2%), for passing period balance and achieving to 5 ° C, the samples were frozen in the 0.25 ml straws. After thawing the characteristics of sperm motility, viability and integrity of the sperm membrane were evaluated. According to the results the total motility in the treatment of 1% was significantly higher than other treatments and the control group (p<0/05). The progressive motility was not significantly different among 0.5 and 1% treatments with control group (p<0/05), although 1% treatment was higher than the control group, but this increasing was not significant. Also a significant decline was observed between 1.5 and 2% treatments than 0.5% and 1% treatment with the control group (p<0/05). A significant increasing was observed in viability between 1% treatment and all other control and treatment groups (p<0/05). The results health membrane show not significant difference among all treatments with control group (p>0/05). It is concluded that supplementation of RJ in freezing extender can improve the cryosurvival rate in Mahabadi bucks, sperm.
