In this study, the effects of CuO/water nanofluid on the heat transfer coefficient, pressure drop and Nusselt number were experimentally investigated. The experiment was at constant heat flux and turbulent flow conditions. Firstly, 0.03%, 0.1%, 0.3%, 0.5% and 0.7% volume fraction of copper oxide nanoparticles with the average size of 50 nm were dispersed in water by ultrasonic agitation. Then the convective heat transfer coefficients and Nusselt number evaluated. Experimental results of nanofluid convective heat transfer were compared with three correlations at turbulent regime. The predicted Nusselt number exhibits good agreement with Gnielinski′s correlation. The results showed by increasing the nanoparticle volume fraction, the convective heat transfer coefficient increased significantly. Also, the nanofluid caused the pressure drop increases than to the base fluid. Using regression, a new correlation has been suggested for the Nusselt number.