This research investigates the influence of thulium (Tm) impurity on the thermoluminescence properties of lithium tetraborate (Li2B4O7 or LTB) nanoparticles synthesized by the combustion method. First, X-ray diffraction pattern (XRD) was used to check the structure and size of nanoparticles made without and with thulium impurity. This analysis confirms the formation of the LTB nanoparticles. Then, the scanning electron microscope (SEM) equipped with EDAX was used to investigate the shape and morphology of the samples and also the existence of Tm (thulium impurity) in the sample. The samples were exposed to 60Co gamma rays ranging from 100Gy to 1000Gy, and their thermoluminescence curves were investigated. The analysis focused on samples that were contaminated with thulium as well as those without impurities. Dose-response curve was drawn and analyzed for both types of samples (with and without impurity). The results show that adding thulium impurity increases thermoluminescence intensity and creates a linear response curve.