A simple, rapid and accurate chemiluminescent method is described for the determination of iproniazid. The proposed method is based on the chemiluminescence (CL) generated during the oxidation of luminol by trichloroisocyanuric acid (TCCA) and sodium dichloroisocyanurate (SDCC) in alkaline medium. It was found that iproniazid could greatly enhance the CL intensity of the mentioned reactions. The detection limits (3σ) were 8.0 and 76 ppb of iproniazid for the TCCA-luminol and SDCC-luminol systems, respectively. The relative CL intensity was linear with the iproniazid concentration in the range of 50-1000 and 1000-2000 ppb for TCCA-luminol system, and 100-2000 ppb for SDCC-luminol system. The method was applied to the determination of iproniazid in a synthetic sample as well as in human serum with acceptable accuracy and precision.
