The Sphecidae (Latreille, 1802) family (braided waist or digger wasps) from Hymenoptera consist of 4 subfamilies, 20 genera and about 660 species in the world. The family members are different in size (from 1.8-30 mm) wasps with distinct abdominal petiole in various color. They have important role as pollinator, plant pests, predator and parasitic in natural environments. 67 species of this family has been reported from Iran. The bees have a wide distribution almost in arid and semi arid areas of world. They have wide distribution in different habitats of Iran and almost live in pastures and arid regions. Since it is not always possible to classify animal using just morphological characters, so, in this research a new systematic approach was used by utilizing ecology, morpho-anatomy, biological interactions for more accurate taxonomy and biological fauna of the family members. In this research 6 species of this family (Ammophila heydenii Dahlbom 1845, Chalybion femoratum Fabricius 1782, Chalybion flebile Lepeletier de saint-fargeau, 1845, Sceliphron destillatorium Illiger 1807, Sphex flavipennis Fabricius 1793, Podalonia tydei Guillou 1841) reported from Golpayegan, Isfahan Province for first time. 10 Populations of 6 species of Sphecidae family were collected from different parts of Golpayegan and identified by using available references. 36 qualitative and quantitative morpho-biometrical characters were identified and studied using suitable equipments. Data were coded and analyzed by principal component analysis (PCA) test using the SPSS. Then cluster were performed. Statistical analysis of morpho-biometrical data showed that U-shaped groove in dorsal surface propodeum, Antenna color, second sub marginal cell length of front wing to basal veinlet (basal veinlet/anterior veinlet), return veins connection to sub marginal cells of front wing, teeth number in front and middle legs nails, teeth arrangement in front and middle legs nails, pulvilli between nails, foot co
