Due to huge number of applications of polymer-noble metal nanocomposites, a simple synthesis route for the design of polypyrrole nanotubes in the presence of Pd nanoparticles (PPy-Pd) has been reported in this paper. In this method synthesized Pd nanoparticles using rosemary extract, as reducing agent, combined with a reverse cylindrical micelle containing pyrrole monomer and an aqueous FeCl3 solution to produce nanotube-shaped PPy-Pd composite. The synthesized nanotube PPy-Pd composite was studied using different characterization techniques such as FTIR, XRD, SEM and TEM. The antibacterial activity of the synthesized nanocomposite was evaluated against clinical isolates of Gram-positive (Bacillus spp. and Staphylococcus aureus) and Gram-negative (Escherichia coli and Klebsiella spp.) bacteria. The Kirby-bauer method was accomplished for determination of zone of inhibition. The obtained results exhibited that antibacterial activity of the nanocomposite was improved compared with pure polypyrrole due to existence of Pd nanoparticles.