The genus Muscari Mill. (Hyacinthaeae) including about 50 apecies and they are garden and ornamenta plants. Muscari neglectum species specifically distributed in central of Iran. genetic diversity of 100 genotype of muscarineglectum were examined using SCOT markers, 10 primers of SCoT markers showed sharp and appopriate repeatable bands. The primers produced between 10 to 23 bands. Totally the markers produces 164 amplification products, out of which 164 bands were polymorphic. The results showed that polymorphism information content (PIC) of primers was 0.246. Cluster analysis based on unweights Pair Group Method with Arithmetic mean (UPGMA) revealed genetic coefficient ranged from 0.22 to 0.84. our result indicated wide range of genetic diversity across the studied accessions of Muscari neglectum in central region of Iran.