Pistachio (Pistacia vera L.) is considered to be the most widely cultivated species in the genus Pistacia and regarded as the most probable ancestral species within the genus. As one of the oldest nut crops in human history, pistachio nuts have a high nutritional value and are commercially important. In the present study, the genetic variation of pistachio genotypes was investigated by nuclear SSR markers. The transferability of the SSR markers was high across genotypes, thus, flanking regions of the SSRs are conserved in the studied genotypes. High level of polymorphism was detected among the studied genotypes. The seven SSR primer pairs generated a total of 18 alleles that 13 of them were polymorphic among the genotypes. The range of the polymorphic alleles was 1 (for Ptms9, Ptms40, Ptms41, and Ptms42) to 5 (for Ptms7 locus) with an average of 2.57. The amplified allele sizes ranged from 120 to 250bp. Pair-wise genetic similarity coefficients varied from 0.20 to 0.75. The UPGMA dendrogram differentiated the genotypes into two major clusters. The present results may be used for the conservation, core collection and future breeding of the pistachio.
